Biocompatibility and nanotoxicity of Graphene oxide and its use as tissue growth template

Giulia FIORAVANTI, Università degli Studi dell'Aquila

In recent years, graphene-based materials have attracted tremendous interest in many different fields including biomedicine, due to their unique properties. Due to the inability of the nervous system to regenerate, biomaterials that support neurons growth and differentiation are of great interest for neurodegenerative disease, especially in cases of damages to the central and peripheral nervous system. In combination with improving stem cell technology, appropriate materials for directing stem cell differentiation have become critical for tissue regeneration. The main strategies developed to apply carbon-based materials to tissue engineering and cell differentiation are divided in suspension or coating techniques, where the first ones leave open the question about the potential toxicity of the dispersed materials. In this presentation we will show the effect of graphene oxide (GO) and reduced graphene oxide (rGO) films on the differentiation of a human neuroblastoma SH-SY5Y cell line, which was an ideal model used to study neuronal differentiation and disease in vitro. As prepared GO films and thermally reduced ones have been deposited and fully characterized. Partially reduced GO films significantly enhanced the differentiation of SH-SY5Y (by evaluating neurite length), providing a promising application for future regenerative medicine.

 
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